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KMID : 1377020120090050259
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Tissue Engineering and Regenerative Medicine
2012 Volume.9 No. 5 p.259 ~ p.264
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Osteoblast differentiation of umbilical cord blood-derived mesenchymal stem cells and enhanced cell adhesion by fibronectin
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Naveen Kumar Mekala
Rama Raju Baadhe
Sreenivasa Rao Parcha
Prameela Devi Y
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Abstract
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Mononuclear cells isolated from human umbilical cord blood were differentiated in to spindle shaped fibrous mesenchymal stem cells (MSCs) in DMEM with 10% fetal bovine serum. These progenitor cells were further examined for their ability to differentiate into osteoblasts by culturing them in osteogenic differentiation media (Stem Pro®). Calcium mineralization assay, alizarin red S, von kossa staining confirmed that MSCs from cord blood were capable of mineralization when they were cultured in osteogenic medium. The adhesion studies shown that MSCs have greater affinity towards fibronectin (FN) coated dishes, compared to albumin (Alb) coated dishes due to CD29and CD44 surface receptors.
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KEYWORD
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cord blood cells, fibronectin, mesenchymal stem cells, osteogenic differentiation, tissue engineering.
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